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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Inflamm Bowel Dis. 2010 Aug 18;17(3):720–731. doi: 10.1002/ibd.21419

Figure 2. Functional analysis of the NHE2 promoter in C2BBe1 cells and identification of TNF-α-responsive region.

Figure 2

A: NHE2 promoter activity is decreased in response to TNF-α. C2BBe1 cells were transiently transfected with the NHE2 promoter-luciferase constructs p−1051/+150 and p−415/+150 followed by incubation without or with TNF-α for 24 h. Luciferase activity was determined as described in Materials and Methods and is presented relative to the normalized activity of the promoter-less pGL2-basic construct. B: Identification of TNF-α responsive region. A series of the 5′-deletion NHE2 promoter reporter constructs were transiently transfected in C2BBe1 cells, treated with or without TNF-α and processed as described above. Values shown are means ± S.E. from three separate experiments performed in triplicates. Significant differences from the control values are shown (*, P < 0.05).