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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Inflamm Bowel Dis. 2010 Aug 18;17(3):720–731. doi: 10.1002/ibd.21419

Figure 3. The effects of TNF-α on the IκB-α, p50 and p65 proteins in C2BBe1 cells.

Figure 3

Cytoplasmic and nuclear proteins were prepared from untreated cells or cells treated with TNF-α for indicated time intervals. Twenty μg protein per sample was subjected to 10% SDS-PAGE and transferred onto PVDF membrane. IκB-α in cytoplasmic fraction (Figure A), and p50 and p65 in nuclear fraction (Figure B) were detected using anti-IκB-α, or anti-p50 and anti-65 antibodies, respectively. As a loading control, the blots were re-probed for tubulin or actin using mouse monoclonal antibodies.