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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Inflamm Bowel Dis. 2010 Aug 18;17(3):720–731. doi: 10.1002/ibd.21419

Figure 6. Overexpression of p50 and p65 attenuate NHE2 promoter activity in C2BBe1 cells.

Figure 6

A: Proliferating C2BBe1 cells were co-transfected with p50 and p65 expression vectors (0.5 μg) individually or simultaneously along with p−1051/+150 NHE2 promoter reporter construct and treated with TNF-α. Overexpression of p50 and p65 inhibited the NHE2 promoter activity ~ 40% and 60% individually and ~ 65% in combination. B: Effect of TNF-α on NHE2 promoter activity in cells over-expressing p50 and p65. C: p50 and p65 stimulate p-NF-κB-luc reporter activity. C2BBe1 cells were co-transfected with p50 and p65 expression vectors individually or simultaneously along with p-NF-κB-luc construct. D: TNF-α and EGF regulate NHE2 promoter activity via discrete pathways. C2BBe1 cells were stably transfected with NHE2 promoter construct, p−890/+150 and then allowed to grown for 12 days before serum starvation and treatment with TNF-α (20 ng/ml), EGF (100 ng/ml) or both. Luciferase activities were determined after 24 h and analyzed in comparison to untreated cells. The data shown are representative of at least three separate experiments performed in triplicates. *, P < 0.05; **, P < 0.001.