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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Inflamm Bowel Dis. 2010 Aug 18;17(3):720–731. doi: 10.1002/ibd.21419

Figure 7. TNF-α-induced repression of NHE2 promoter is mediated via NF-κB signaling pathway.

Figure 7

C2BBe1 cells were transiently transfected with the p−1051/+150 NHE2 promoter reporter constructs and serum-starved in DMEM containing 0.5% FBS overnight. Cells were incubated with CAPE (25 μM) (A), and LC (5 μM) (B) for 1 h prior to addition of TNF-α then TNF-α was added and incubation continued for 24 h. 48 h post-transfection, cells were harvested and luciferase and β-galactosidase activities were determined. Data shown are mean± S.E. of least three separate experiments performed in triplicates. *, P <0.05.