Table 4.
Scatchard plot data on Ethidium Bromide binding pattern
| Brain regions | Control | BD |
|---|---|---|
| Thalamus | 0.63±0.09 | 0.44±0.07* |
| Medulla | 0.61±0.11 | 0.32±0.15* |
| Pons | 0.38±0.1 | 0.12±0.03* |
| Cerebellum | 0.53±0.16 | 0.20±0.04* |
| Hippocampus | 0.18±0.06 | 0.15±0.1 |
| Temporal lobe | 0.39±0.08 | 0.32±0.03* |
| Occipital lobe | 0.45±0.12 | 0.40±0.08 |
| Parietal lobe | 0.45±0.15 | 0.51±0.11 |
| Hypothalamus | 0.32±0.08 | 0.26±0.13 |
| Frontal cortex | 0.44±0.1 | 0.25±0.03* |
Bound Ethidium Bromide (EtBr) per base pair (bp) of DNA from BD and control samples were calculated using Scatchard plots. Values are as mean of number of EtBr molecules bound per base pair of DNA±SD. The values with asterisk
(*)
are significantly different from the corresponding control values at P<0.02 of eight control and ten BD DNA samples. The statistical significance was calculated using Student’s t test.