Table 3.

Kinetic data for unnatural base pair extension via dCTP incorporation with dMMO2 derivatives in the primer.^[a]

5′–d(TAATACGACTCACTATAGGGAGAY)
3′–d(ATTATGCTGAGTGATATCCCTCTXGCTAGGTTACGGCAGGATCGC)
X=d5SICS
Y	kcat (min−1)	KM (μM)	kcat/KM (M−1 min−1)
dMMO2[b]	6.4 ± 1.1	3.4 ± 0.3	1.9 × 106
d5FM	3.5 ± 0.11	0.63 ± 0.1	5.5 × 106
d5ClM	4.2 ± 0.8	2.0 ± 0.06	2.0 × 106
d5BrM	5.3 ± 0.9	2.1 ± 0.07	2.6 × 106
d5IM	4.3 ± 1.3	15 ± 0.3	2.9 × 105
d5MeM	2.4 ± 0.06	1.7 ± 1.18	1.4 × 106
d5PrM	2.6 ± 0.52	9.6 ± 1.78	2.7 × 105
d5SICS[b]	nd[c]	nd[c]	<1.0 × 103

^[a]see Experimental section for details.

^[b]Reference 5.

^[c]Reaction was too inefficient for k_cat and K_M to be determined independently.