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. 2010 Oct;162(1):146–155. doi: 10.1111/j.1365-2249.2010.04212.x

Fig. 3.

Fig. 3

T cells but not natural killer (NK) cells of patient 1119 produce fewer cytokines compared to single cord blood controls, and the minor unit produces fewer cytokines than the major, with the exception of interleukin (IL)-2. Analyses were performed with blood samples from months 42, 43 and 55 post-transplantation for patient 1119. Peripheral blood mononuclear cells (PBMCs) were stimulated with (a,c) ionomycin and phorbol 12-myristate 13-acetate (PMA) and incubated for 8 h, or (b,d) mitogen or K562 for 4 h, at 37°C and stained with indicated antibodies. (a) The cytokine production by T cells in response to ionomycin and PMA of patient 1119 (n = 3) compared to single cord blood controls (n = 4). (b) Interferon (IFN)-γ production and CD107a up-regulation in NK cells of patient 1119 (n = 3) compared to single cord blood controls (n = 4). (c) Comparison of cytokine production by T cells of the two units in patient 1119 (n = 2). (d) IFN-γ production and CD107a up-regulation in NK cells of the two units in patient 1119 (n = 2).