Table 1.
Specific primers used in real-time polymerase chain reaction analysis.
| Name | Primer | Sequence (5′→ 3′) |
|---|---|---|
| β-actin | FW | TGTCCACCTTCCAGCAGATGT |
| RV | AGCTCAGTAACAGTCCGCCTAGA | |
| CVB3 | FW | ATCAAGTTGCGTGCTGTG |
| RV | TGCGAAATGAAAGGAGTGT | |
| IFN-γ | FW | TCAAGTGGCATAGATGTGGAAGAA |
| RV | TGGCTCTGCAGGATTTTCATG | |
| IL-4 | FW | ACAGGAGAAGGGACGCCAT |
| RV | GAAGCCCTACAGACGAGCTCA | |
| IL-6 | FW | TTCCATCCAGTTGCCTTCTTG |
| RV | TTGGGAGTGGTATCCTCTGTGA | |
| MCP-1 | FW | CGGAATTCGCCACCATGCAGGTCCCTGTCAT |
| RV | GCTCTAGACTAGTTCACTGTCACACT | |
| MIP-2 | FW | CACTTCAGCCTAGCGCCAT |
| RV | AGGTCAGTTAGCCTTGCCT | |
| TNF-α | FW | GACGTGGAACTGGCAGAAGAG |
| RV | GCCACAAGCAGGAATGAGAAG |
CVB3: Coxsackievirus B3; IFN: interferon; IL: interleukin; MCP: monocyte chemoattractant protein; MIP: macrophage inflammatory protein; TNF: tumour necrosis factor. FW: forward; RV: reverse.