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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: J Cell Physiol. 2009 Nov;221(2):402–411. doi: 10.1002/jcp.21869

Figure 1. Cytokines do not significantly affect MMP-9 expression in CECs with or without cholera toxin.

Figure 1

Zymographic analysis of MMP-9 secreted into the media by CECs following TGF-β1, TGF-β2, IL-1β, HB-EGF, EGF, and FGF-2 treatments (10 ng/mL) with or without cholera toxin (30 ng/mL), a known up-regulator of cAMP. Treatment with an EGFR inhibitor, AG1478 (50 ng/ml), had no effect on MMP-9 expression with or without EGF treatment. Similarly, cholera toxin failed to affect MMP-9 expression in conjunction with any of the various cytokines. PMA (1μM) was used as a positive control for MMP-9 stimulation. A human MMP-9 standard was run in parallel for sizing of the rabbit MMP-9 zymogram bands.

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