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. Author manuscript; available in PMC: 2011 Nov 18.
Published in final edited form as: Neuron. 2010 Nov 18;68(4):654–667. doi: 10.1016/j.neuron.2010.09.034

Figure 7. PGRN levels are increased in mice lacking Sortilin.

Figure 7

(A) WT and GRN−/− mouse brain lysate was analyzed by anti-PGRN immunoblot.

(B) Tissue lysate collected from the cerebral cortex of 7-month-old mice was subjected to SDS-PAGE and anti-PGRN immunoblot. Two PGRN bands are seen at 78KDa (band A) and at 73KDa (band B); both bands increase in the Sort1−/− samples compared to WT.

(C) Quantification of PGRN level (bands A, B and A+B) in the cortical lysate, normalized to GAPDH immunoreactivity. Sort1−/− n = 4 mice, WT n = 5. Mean ± sem, **, P < 0.01, one-way ANOVA.

(D) Serum samples from 7-month-old Sort1−/− and WT mice were collected, stripped of albumin and IgG and immunoblotted for PGRN and transferrin. Serum levels of PGRN were increased in the Sort1 −/− compared to littermate WT.

(E) Quantification of PGRN level in the serum normalized to transferrin level. For both genotypes, n = 4 mice. Mean ± sem, **, P < 0.01, one-way ANOVA.

(F) Serum samples from 2-month-old mice were obtained from the indicated genotypes and analyzed by anti-PGRN immunoblot, with anti-thrombin III immunoblot as a loading control.

(G) Quantification of PGRN level in the serum normalized to anti-thrombin III level. For all genotypes, n = 4 mice. Mean ± sem; *, P < 0.05; **, P < 0.01; one-way ANOVA.