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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Transl Res. 2010 Dec;156(6):335–349. doi: 10.1016/j.trsl.2010.09.003

Figure 3. A) Arginase-1 (Arg1) protein expression in whole lung homogenate by Western blot, B) Total arginase enzyme activity in whole lung homogenate, and C) Correlation between total arginase enzyme activity and Arg1 protein expression.

Figure 3

A) Simvastatin (40 mg/kg) reduced Arg1 protein expression by 59.4% (*p<0.05 by 1-way ANOVA). Co-treatment with MA (20 mg/kg) reversed the inhibitory statin effect on Arg1 expression, but this was not statistically significant (p=NS by 1-way ANOVA, p=0.057 by t-test). Each treatment group had between 8–12 mice.

B) Simvastatin (40 mg/kg) reduced total arginase enzyme activity by 57% in the OVA group (*p<0.005 by 1-way ANOVA). Co-treatment with MA (20 mg/kg) reversed the statin effect (not significant by 1-way ANOVA, but significant by t-test (#p=0.0275)). Each treatment group had between 6–9 mice.

C) Arg1 protein expression is represented by protein band intensity (measured by Western blots of whole lung homogenates). Total arginase enzyme activity correlated positively with Arg1 protein expression (r2=0.70, p<0.0001). The dotted lines above and below the best fit line represent the 95% confidence interval. Each dot represents one mouse.