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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Transl Res. 2010 Dec;156(6):335–349. doi: 10.1016/j.trsl.2010.09.003

Figure 7. A) Concentration of TGFβ1 in whole lung lavage, B) Whole lung lavage Absolute Macrophage Counts, and C) TGFβ1 immunohistochemical staining of lung and inflammatory cells.

Figure 7

A) The group 6OVA+EtOH had significantly higher TGFβ1 concentration than any of the FA controls (*p<0.005 by 1-way ANOVA). Simvastatin (40 mg/kg) reduced TGFβ1 concentration in lung lavage by 60.4% in the OVA group (p=NS by 1-way ANOVA or t-test). Co-treatment with MA (20 mg/kg) did not reverse this inhibitory statin effect on TGFβ1. Each treatment group had between 5–6 mice.

B) Simvastatin (40 mg/kg) treatment decreased lung lavage absolute macrophage count by 48.3% (*p<0.005 by 1-way ANOVA) in OVA exposed animals. Co-treatment with MA (20 mg/kg) reversed the simvastatin inhibitory effect (p=NS by 1-way ANOVA, but #p=0.0029 by t-test). Each treatment group had between 10–17 mice.

C) By qualitative immunohistochemical assessment, macrophages appear to be the main inflammatory cells responsible for TGFβ1 production under OVA-induced allergic inflammation (image shows the 6OVA+EtOH treatment group: representative slide where TGFβ1 stains brown seen at x400 magnification). Arrows represent inflammatory cells with little-to-no staining for TGFβ1 (likely eosinophils or lymphocytes). Arrow heads represent macrophages that stain densely brown for TGFβ1.