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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: J Mol Biol. 2008 Sep 26;382(1):179–187. doi: 10.1016/j.jmb.2008.07.004

Figure 3. Transition-state model of switch I, and its interactions in the AlF4 complex.

Figure 3

(a) Model building of the switch 1 region in the transition state. The structure of Giα1 (PDB 1AS2), based on which the model was built, is shown in a wheat cartoon representation. The switch 1 region, the GDP nucleotide and γ phosphate (liberated, but trapped) are shown in red. A large insertion domain is present in switch 1 of Giα1. The G-domain of eEF2 (from PDB 1N0U) is superimposed on Giα1 and shown in slate-blue. Modelling of the transition state conformation of eEF2 is based on this conformation. The small helix of switch 1, which is generally observed in translation GTPases, docks into an additional density observed in our 80S:eEF2:GDP:AlF4− complex.

(b) Interaction of the transition state switch I conformation (green) in the AlF4 complex with the junction of small subunit rRNA helices 8 (blue) and 14 (red) shown in stereo representation. The short helix of the switch I structure (marked with asterisk) is placed close to the conserved tetraloops of the rRNA helices.