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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Pediatr Dev Pathol. 2010 Jan;13(1):1–8. doi: 10.2350/08-03-0443.1

Table I.

Immunohistology and mutational analysis of carcinogen induced rat nephroblastomas and mesenchymal tumors

Sample no. Carcinogen Histology Immuno-reactivity Score DNA Sequencing Ctnnb1 exon 3
ca /na
normal rat kidney
NK none normal rat kidney + wtd
primary nephroblastomas
N1 ENUb Triphasic ++ + wt
N2 ENU Triphasic, predominant blastemal ++ + wt
N3 ENU Triphasic, predominant blastemal + + wt
N4 ENU Triphasic, predominant blastemal + wt
N5 ENU Triphasic, predominant blastemal/stromal ++ wt
N6 ENU Triphasic, weak differentiated + + wt
N7 ENU Triphasic, predominant blastemal + + wt
N8e ENU Triphasic ++ wt
N9e ENU Triphasic ++ wt
N10e ENU Triphasic + wt
N11e ENU Triphasic, mainly blastemal ++ + wt
N12e ENU Triphasic + + wt
N13e ENU Triphasic + + wt
N14e ENU Triphasic + wt
N15e ENU Triphasic wt
N16 DMN-OMec Triphasic, predominant blastemal + + wt
N17 DMN-OMe Triphasic, predominant stromal ++ + wt
N18 DMN-OMe Triphasic, predominant stomal, anaplastic ++ + wt
transplanted nephroblastomas
HT1e ENU Triphasic, predominant blastemal with focal epithelium ++ + wt
HT2e ENU Triphasic, predominant bastemal ++ + wt
HT3e ENU Triphasic, predominant blastemal + + wt
HT4e ENU Triphasic, predominant blastemal + wt
HT5e ENU Triphasic, predominant mesenchymal + wt
HT6e ENU Triphasic, predominant blastemal/stromal + wt
HT7e ENU Triphasic + wt
cell lines
ENU-T1 ENU adherent cells wt
CC531 adherent cells Codon 41, ACC→ATC
Thr→Ile
a

c/n, cytoplasmatic/nuclear staining

b

ENU, transplacental ENU treated animals

c

DMN, neonatal DMN-Ome treated animals

d

wt, wild type

e

DNA extracted by microdissection