Table I.

Immunohistology and mutational analysis of carcinogen induced rat nephroblastomas and mesenchymal tumors

Sample no.	Carcinogen	Histology	Immuno-reactivity Score		DNA Sequencing Ctnnb1 exon 3
			ca	/na
normal rat kidney
NK	none	normal rat kidney	+	−	wtd
primary nephroblastomas
N1	ENUb	Triphasic	++	+	wt
N2	ENU	Triphasic, predominant blastemal	++	+	wt
N3	ENU	Triphasic, predominant blastemal	+	+	wt
N4	ENU	Triphasic, predominant blastemal	+	−	wt
N5	ENU	Triphasic, predominant blastemal/stromal	++	−	wt
N6	ENU	Triphasic, weak differentiated	+	+	wt
N7	ENU	Triphasic, predominant blastemal	+	+	wt
N8e	ENU	Triphasic	++	−	wt
N9e	ENU	Triphasic	++	−	wt
N10e	ENU	Triphasic	+	−	wt
N11e	ENU	Triphasic, mainly blastemal	++	+	wt
N12e	ENU	Triphasic	+	+	wt
N13e	ENU	Triphasic	+	+	wt
N14e	ENU	Triphasic	+	−	wt
N15e	ENU	Triphasic	−	−	wt
N16	DMN-OMec	Triphasic, predominant blastemal	+	+	wt
N17	DMN-OMe	Triphasic, predominant stromal	++	+	wt
N18	DMN-OMe	Triphasic, predominant stomal, anaplastic	++	+	wt
transplanted nephroblastomas
HT1e	ENU	Triphasic, predominant blastemal with focal epithelium	++	+	wt
HT2e	ENU	Triphasic, predominant bastemal	++	+	wt
HT3e	ENU	Triphasic, predominant blastemal	+	+	wt
HT4e	ENU	Triphasic, predominant blastemal	+	−	wt
HT5e	ENU	Triphasic, predominant mesenchymal	−	+	wt
HT6e	ENU	Triphasic, predominant blastemal/stromal	−	+	wt
HT7e	ENU	Triphasic	+	−	wt
cell lines
ENU-T1	ENU	adherent cells			wt
CC531		adherent cells			Codon 41, ACC→ATC Thr→Ile

^ac/n, cytoplasmatic/nuclear staining

^bENU, transplacental ENU treated animals

^cDMN, neonatal DMN-Ome treated animals

^dwt, wild type

^eDNA extracted by microdissection