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. 2010 Sep 16;8(3):225–235. doi: 10.1016/j.chom.2010.08.002

Figure 1.

Figure 1

C. albicans Infection of TR146 Epithelial Cells Activates NF-κB and MAPK Signaling

(A) Increasing phosphorylation of IκB-α and p65 from 5 min postinfection onward. Bands are shown relative to unphosphorylated protein and α-actin loading control.

(B) Transcription factor DNA binding activity of p65 after C. albicans infection as measured by TransAM ELISA.

(C) Increased phosphorylation of MEK1/2, ERK1/2, JNK, and p38 after 15 min postinfection, with further phosphorylation at 2 hr, indicating a biphasic response. Also, phosphorylation of MKP1 only at 2 hr postinfection, indicating stabilization of the MAPK response.

(D) Decreasing levels of p38 and JNK phosphorylation after 2 hr infection, matching with increasing levels of MKP1 phosphorylation.

(E) Levels of DNA binding activity (absorbance values) of AP-1 transcription factor members in resting TR146 cells.

(F) Changes in binding activity of c-Fos, c-Jun, Elk1, and MEF2 after infection; data represented as fold change relative to resting cells. A C. albicans:epithelial cell MOI of 10:1 was used.

Data are (A, C–E) representative of three independent experiments or mean of at least three (B) or six (F) independent experiments ± SEM. ∗p < 0.05, ∗∗p < 0.01. (Two bands are seen for ERK1/2 and JNK, as two different proteins make up these complexes). See also Figure S1.