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. Author manuscript; available in PMC: 2010 Nov 24.
Published in final edited form as: Clin Cancer Res. 2009 Mar 10;15(6):1947–1953. doi: 10.1158/1078-0432.CCR-08-2332

Fig. 5.

Fig. 5

Effect of oral feeding of GTP to TRAMP on downstream IGF signaling. Oral GTP (0.1% in drinking water ad libitum) was initiated in animals at ages representing different stages of the disease as described in the text. A, at age 32 wk, the dorsolateral prostate was dissected and tissue lysates were prepared and subjected to immunoblotting for PI3K/p85, pAkt, and phosphorylated ERK. Five samples from each group were analyzed by immunoblotting under identical conditions; however, only two representative bands from each are shown. B, bands for pAkt were subjected to densitometric analysis to ascertain quantitatively the change compared with control. Mean ± SD relative density of five bands from each group normalized to whole Akt.