A) MAGEA4-specific CTL were stimulated and expanded in vitro by peptide-pulsed DC. To confirm specificity of the CTL lines, IFN-γ ELIspot analyses were performed against a MAGEA4 PepMix and compared to an irrelevant PepMix (Aspf16) and CTL alone, demonstrating MAGEA4 peptide specificity. B) MAGEA4-specific CTL were HLA-A2-restricted as demonstrated by co-culture with the HLA-A2+ cell line, HDLM2, where cell lysis was observed when the tumor cells were cultured with CTL and 1 μM decitabine, but not without CTL or with an MHC-mismatched MAGEA4 CTL line. C) Histogram which compares HDLM2 viability (PI) indicating lysis of HDLM2 when cultured with MAGEA4-specific CTL (gray lines) or without CTL (black lines) in the presence of decitabine.