Figure 5.

Epidermal growth factor + EPO infusions led to tissue regeneration in the motor cortex after focal stroke. (A,B) Experimental paradigm. Devascularizing lesion on day 0 was followed by EGF and/or EPO infusion via an intraventricular cannula in the contralateral hemisphere beginning on day 7 poststroke. EGF was infused for 7 days followed by EPO for 7 days. (C,D) Dorsal photographs of lesions brains (42 days after stroke), infused with either CSF + CSF (C) or EGF + EPO (D). The stroke produced a chronic cavity whereas treatment with EGF + EPO led to the development of newly generated cortical tissue. (E–H) Coronal cresyl violet-stained sections showing the lesion cavity in a CSF + CSF lesion brain (E) and an EGF + EPO lesion brain (F). The lesion cavity is filled with tissue in the latter brain. The intact hemisphere of the EGF + EPO brain has clear lamination characteristic of motor cortex (G) but there is no obvious organization in the newly generated cortical tissue and a complete absence of a layer I (H) (modified from Kolb et al., 2007).