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. 2010 Aug 24;16(12):3611–3622. doi: 10.1089/ten.tea.2010.0219

FIG. 5.

FIG. 5.

Practicable illustration of the superiority of MCG employing PTCC strategy (“PTCC” samples), in contrast with plain agarose hydrogel without alteration (“Control” samples) in vitro and in vivo. (A) Analysis of the expression of relevant cartilaginous markers at transcriptional level from porcine chondrocytes in PTCC samples and Control samples, adopting agarose hydrogels, by quantitative real-time PCR (n = 3). The values represent the levels of expression relative to that of two internal control genes (HPRT and RPL4). Gray: cells in PTCC samples (n = 3); white: cells in Control samples (n = 3). The values represent the means ± standard deviations. The selected time points of analysis are 3, 10, 18, and 32 days, respectively. (B) Investigation of the secretion of extracellular matrix in PTCC samples and Control samples. The row panels from the top to the bottom illustrate the secretion of GAG and total collagen, respectively, as a function of culture time at 3, 7, 12, 18, 24, and 32 days. *Differences between different constructs at the same culture time are not significant (p > 0.05); **differences between different constructs at the same culture time are significant (p < 0.05).