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. 2010 Nov 24;6(11):e1001210. doi: 10.1371/journal.ppat.1001210

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Kennan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sheep were challenged with the wild type, the three protease mutants and their complemented derivatives in a blind pen trial. The total weighted foot score (TWFS) for individual sheep at week three is shown. The mean and the s.e.m. for each group are shown. Each mutant is significantly different to the wild-type, while the aprV2/aprV2⁺ and aprV2/aprB2⁺ complemented strains are not significantly different to wild-type (P<0.05, n = 8, one-way ANOVA). NC: negative control, not infected with bacteria.