Figure 7. Characterisation of AprV2.C141S derivative.

(A) Degradation of fibronectin by purified AprV2 and AprV2.C141S at 25°C. The degradation of fibronectin over time was assessed by SDS-PAGE analysis. (B) Stern-Volmer plot for iodide quenching of tryptophan in AprV2 and AprV2.C141S. Purified protease was incubated with increasing amounts of KI, and the fluorescence emission intensity was recorded. The lines represent a least squares fit of the experimental data as described previously [49]. The mean and s.e.m from three independent experiments are shown. Note that in these figures AprV2.C141S is shown as C141S.