Table 1. Activity of AprV2, AprB2 and mutants against the elastin like peptide AAPV(n).

Proteases	Vmax (µM.s−1)	K M (mM)	kcat (s−1)	kcat/K M (M−1s−1)
AprV2	0.025±0.001	0.54±0.15	0.025±0.001	46.3
AprB2	0.033±0.001	0.41±0.08	0.033±0.001	80.5
AprV2.C141S	0.020±0.001	0.82±0.24	0.020±0.001	24.4
AprV2.Y92F	0.034±0.002	1.12±0.18	0.034±0.002	30.4
AprV2.Y92L	0.045±0.001	0.95±0.12	0.045±0.001	47.4
AprV2.Y92A	0.038±0.001	0.83±0.08	0.038±0.001	45.8
AprV2.Y92D	0.041±0.001	0.81±0.08	0.041±0.001	50.6

C141S is a substitution derivative of AprV2 in which one disulphide bond has been removed. AprB2 is equivalent to AprV2.Y92R. Experiments were conducted at 25°C using 1 µM purified protease.