Figure 5. The PHD fingers of Lid bind specific forms of methylated histone tails.

GST fusion proteins of Lid's PHD1 (top panel), PHD2 (2^nd panel) and PHD3 (3^rd panel) were tested for binding to biotinylated histone peptides of histone H3 amino acids 1–21, 21–44 and histone H4 1–21. In addition, peptides mono, di or tri methylated at histone H3 K4, K9 and K27 were also tested for their ability to bind these PHD fingers.