Table 1. Intracellular concentrations of target metabolites from cells during transition from succinate growth to methanol growth.
| zero | 10 min | 20 min | 30 min | 1 h | 2 h | 3 h | ||
| Pathway | Metabolite | Concentration (mM) | ||||||
| TCA | Suc | 12.2±0.7 | 3.7±2.5 | 2.1±0.7 | 2.9±0.8 | 5.0±0.3 | 2.2±0.3 | 2.3±0.9 |
| Fum | 0.5±0.1 | 0.6±0.1 | 0.5±0.0 | 0.5±0.1 | 0.5±0.1 | 0.5±0.1 | 0.5±0.1 | |
| Mal | 1.6±0.4 | 1.7±0.4 | 1.2±0.2 | 1.3±0.4 | 1.0±0.4 | 0.9±0.1 | 1.2±0.4 | |
| 2KG | 1.4±0.3 | 1.5±0.2 | 1.7±0.3 | 1.4±0.4 | 1.5±0.3 | 2.3±0.3 | 2.5±0.4 | |
| Cit | 1.8±0.5 | 2.0±0.4 | 3.3±0.4 | 1.8±0.4 | 2.2±0.6 | 2.1±0.4 | 2.4±0.5 | |
| Pyr | 3.7±0.4 | 4.4±1.2 | 3.9±0.3 | 4.6±0.5 | 5.1±0.7 | 4.8±0.4 | 4.9±0.3 | |
| Serine | Glyc | 1.7±0.3 | 2.5±0.6 | 2.0±0.5 | 2.1±0.3 | 2.0±0.4 | 1.8±0.2 | 2.2±0.7 |
| Ser | 4.0±1.5 | 4.5±1.1 | 4.2±1.0 | 16.7±10.4 | 10.1±6.8 | 3.3±0.8 | 2.9±1.7 | |
| Gly | 0.8±0.1 | 0.8±0.1 | 0.8±0.1 | 0.9±0.2 | 1.0±0.1 | 1.0±0.0 | 1.2±0.1 | |
| PHB | OHB | 3.0±0.2 | 3.3±0.4 | 3.4±0.2 | 3.3±0.5 | 4.4±0.7 | 4.0±0.5 | 4.3±0.9 |
| OHB-CoA | 0.1±0.1 | 0.2±0.1 | NMa | 0.4±0.1 | 0.3±0.1 | NM | 0.3±0.1 | |
| EMC | EtMA | 0.6±0.1 | 0.8±0.1 | 1.1±0.1 | 1.0±0.1 | 1.2±0.1 | 2.1±0.2 | 3.1±0.4 |
| MeSuc | 0.6±0.0 | 0.7±0.1 | 0.8±0.0 | 0.7±0.1 | 0.8±0.0 | 1.7±0.1 | 4.3±0.8 | |
| P-CoA | 0.1±0.1 | 0.1±0.1 | NMa | 0.1±0.1 | 0.2±0.1 | NM | 0.3±0.1 | |
| MeMA | 0.3±0.0 | 0.1±0.1 | NM | 0.1±0.1 | 0.2±0.1 | NM | 0.3±0.1 | |
| Otherb | Ac-CoA | 0.3±0.1 | 0.2±0.1 | NM | 0.2±0.1 | 0.2±0.1 | NM | 0.3±0.1 |
| M-CoA | 0.1±0.1 | 0.3±0.1 | NM | 0.1±0.1 | 0.2±0.1 | NM | 0.2±0.1 | |
| CoA | 2.6±0.2 | 0.7±0.5 | NM | 1.5±0.2 | 1.2±0.3 | NM | 1.4±0.5 | |
| Ala | 7.6±0.7 | 9.4±2.0 | 9.4±1.1 | 14.0±1.5 | 12.9±4.1 | 9.7±0.3 | 10.0±0.6 | |
| Val | 1.0±0.1 | 0.7±0.2 | 0.9±0.1 | 1.4±0.4 | 1.5±0.6 | 1.2±0.1 | 1.3±0.2 | |
| Leu | 0.6±0.0 | 0.6±0.2 | 0.8±0.0 | 0.9±0.1 | 0.9±0.4 | 1.1±0.1 | 1.4±0.3 | |
| Ile | 0.4±0.1 | 0.5±0.1 | 0.6±0.0 | 0.7±0.1 | 0.7±0.1 | 0.5±0.0 | 0.6±0.0 | |
| Pro | 1.3±0.2 | 1.3±0.3 | 1.6±0.1 | 1.6±0.3 | 1.4±0.4 | 1.1±0.2 | 1.0±0.4 | |
| Thr | 3.1±0.4 | 3.4±0.6 | 3.4±0.6 | 6.3±3.4 | 5.2±1.1 | 3.0±0.5 | 3.3±0.7 | |
| Asp | 4.9±1.5 | 4.9±1.3 | 4.9±0.5 | 5.9±1.1 | 6.2±3.1 | 2.2±2.0 | 2.3±2.4 | |
| Phe | 0.3±0.0 | 0.4±0.1 | 0.7±0.1 | 0.5±0.2 | 0.4±0.2 | 0.2±0.1 | 0.2±0.1 | |
Numbers are averages of nine replicates, and standard deviations are listed as ± sd. The abbreviations used in the table are defined as follows: Suc = succinate; Fum = fumarate; Mal = malate; 2KG = 2-ketoglutarate; Cit = citrate; Pyr = pyruvate; Glyc = glycerate; OHB = 3-hydroxybutyrate; OHB-CoA = 3-hydroxybutyryl-CoA; EtMA = ethylmalonate; MeSuc = methylsuccinate; P-CoA = propionyl-CoA; MeMA = methylmalonate; Ac-CoA = acetyl-CoA; M-CoA = malonyl-CoA.
a
NM = not measured.
b
Other refers to measured metabolites that are not depicted in the central metabolic diagrams (Figure 4) or are components of multiple central metabolic pathways.