Figure 2. CPY* variants D1 and D2 are degradation defective.
Wild type and Δpep4 cells expressing CPY* and variants following galactose induction were pulse-labeled for 10 min with [35S]methionine/cysteine and chased for times indicated. Substrate proteins were immunoprecipitated from detergent lysates, separated by SDS-PAGE, and visualized and quantified by phosphorimager analysis. Representative gel scans are shown on the left. The position of substrate proteins and hyperglycosylated species are indicated. Data plots reflect three independent experiments with standard deviations indicated by the error bars.