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. 2010 Nov 24;5(11):e15491. doi: 10.1371/journal.pone.0015491

Table 1. Biophysical characterization of purified [FeFe] hydrogenases produced in Escherichia coli.

HydA1 CpI
H2 Uptake (MV) 251±49 476±39
µmol H2·min−1·mg−1
H2 Evolution (MV) 641±88 1087±146
µmol H2·min−1·mg−1
H2 Evolution (PetF) 41±4 90±10
µmol H2·min−1·mg−1
Iron Content 4.5±0.2 13.2±1.3
Fe atoms·peptide−1
Purification Yield 30±11 7.9±0.8
mg·L−1 of culture

Data are the average from n = 3–6 cultures examined ± standard deviations.

Specific activities of purified hydrogenases were determined using methyl viologen (MV) or PetF ferredoxin as the electron donating or accepting substrates.