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. 2010 Nov 24;5(11):e15515. doi: 10.1371/journal.pone.0015515

Figure 4. Nestin-Cre driver efficiently inactivates Jag1 in V1 and dI6 domains.

Figure 4

(A, B) Immunofluorescence in Jag1f/f;Rosa26YFP/+;NesCre E11.5 embryos shows widespread YFP expression, indicating that most of the cells had undergone Cre-mediated recombination. Since YFP signal fades away during longer fixations, IF with an anti-GFP antibody was used to detect YFP expression. (A′, B′) The two characteristic stripes of Jag1-protein expression are absent in Jag1f/f;Rosa26YFP/+;NesCre embryos. (A″, B″) Merge of YFP and Jag1 expression. Scale bar 50 µm. Strong red signal in B′, B″ is due to erythrocytes.