Table 3.
Averages and Ranges of Thermodynamic Parameters for Single Mismatch-Nearest Neighbor Sequence Combinationsa
| sm positionb | ΔH°SM (kcal/mol) | ΔS°SM (cal/K·mol) | ΔG°37,SM (kcal/mol) | |
|---|---|---|---|---|
| center | average | −12.5 | −43.0 | 0.82 |
| range | (−23.8 – −2.2) | (−74.5 – 8.1) | (−0.64 – 2.26) | |
| off-centerc | average | −4.6 | −16.3 | 0.45 |
| range | (−17.4 – 7.6) | (−54.0 – 23.5) | (−0.79 – 1.61) | |
| 5′-shifted | average | −4.1 | −14.3 | 0.36 |
| range | (−17.4 – 6.4) | (−54.0 – 18.2) | (−0.79 – 1.58) | |
| 3′-shifted | average | −5.1 | −18.2 | 0.54 |
| range | (−15.7 – 7.6) | (−50.2 – 23.5) | (−0.16 – 1.61) | |
a
Averages and ranges are based on the data obtained from TM−1 vs. ln(CT/4) plots of the nine complete sets of data as described in the Materials and Methods. Errors associated with the individual ΔH°SM, ΔS°SM, and ΔG°37,SM values used to calculate the average values listed here are approximately ± 6.3 kcal/mol, ± 19.4 cal/K·mol, and ± 0.31 kcal/mol, respectively.
b
The duplex position of the single mismatch as described in Materials and Methods.
c
The off-center values are an average of the 5′- and 3′-shifted single mismatch data.