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. Author manuscript; available in PMC: 2011 Nov 22.
Published in final edited form as: Small. 2010 Nov 22;6(22):2509–2513. doi: 10.1002/smll.201001341

Figure 4.

Figure 4

NSC alignment and differentiation on combinatorial ECM patterns. A) NSCs on grids of laminin express the neural stem cell marker, nestin (purple) on Day 2 after seeding, thus confirming that the NSCs are undifferentiated. B) NSCs stained for actin (green) show extensive spreading and cell–cell interactions on grid patterns of laminin on Day 2 after seeding, confirming that the NSCs, while still in the undifferentiated state, extensively interact with each other. C) SEM image of NSCs on Day 2 after seeding, showing the early alignment and extension of processes on grid patterns of laminin. D) NSCs previously shown to extend and grow on the grid patterns of laminin undergo neuronal differentiation and express the neuronal marker synapsin (pseudocolored yellow) on Day 6 after seeding. Scale bars: 20 μm.