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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Exp Cell Res. 2010 Oct 14;317(1):107–116. doi: 10.1016/j.yexcr.2010.10.005

Figure 6. cIAP-1 is cleaved by caspase-8 in cell-free system and in vivo.

Figure 6

(A) Recombinant human cIAP-1 was incubated with human recombinant active caspase 8 (1 U) or human recombinant active caspase 3 (0.5 unit), in the presence or absence of Q-VD-OPH (100 μM) for 30 minutes at 37°C and analyzed by immunoblot. Newly generated fragments are indicated by the arrows (left panel). As controls, recombinant human Bid or PARP were incubated with human recombinant active caspase 8 (0.25-1 U) or human recombinant active caspase 3 (0.5 unit), respectively, for 30 minutes at 37°C and analyzed by immunoblot (right panel). (B) HuH-7 cells were incubated with TRAIL (20 ng/ml) and MG132 (10 μM; 1 hr pre-incubation), in the presence or absence of Q-VD-OPH (5 μM) or z-IETD-fmk (10 μM). Cell lysates were analyzed by immunoblot for cIAP-1 and actin (loading control). The asterisk (*) indicates a fragment of approximately 32 kDa generated both in vivo and in the cell-free system.