Fig.3.

Rabbit anti-AβN11(pE) antibodies bound to Aβ oligomers in Western Blot. Oligomers were prepared and analyzed as described in Materials and Methods section. (A) Anti-AβN11(pE) antibodies were used to detect AβN3(pE) and AβN11(pE). A monoclonal anti-Aβ antibody 4G8 was used as a control. (B) Rabbit anti-AβN11(pE) antibodies were used to detect oligomers of Aβ1-42. A monoclonal anti-Aβ antibody (4G8) binding to a central part of Aβ1-42 was used as a control. No binding of anti-AβN11(pE) antibodies to a monomer of Aβ1-42 was observed although a control antibody (4G8) detected it in the same preparation. Migration of the molecular mass standards is indicated.