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. Author manuscript; available in PMC: 2011 May 18.
Published in final edited form as: Oncogene. 2010 Aug 23;29(46):6115–6124. doi: 10.1038/onc.2010.350

Figure 3. TNC knockdown inhibits pulmonary metastasis of melanoma cells.

Figure 3

(A) Representative lungs showing decreased metastasis in mice injected with TNC-shRNA-expressing (sh_TNC_A or sh_TNC_C) versus control (sh_Cont) cells. 2×105 WM3734 melanoma cells were injected intravenously into NOD/SCID IL2Rγnull mice. Mice were sacrificed after 8 weeks and the lungs harvested. (B) Representative lungs stained with H&E. Scale bar = 5 mm (C) The percentage of total area occupied by lung tumor metastases was measured based on H&E stained sections. Data are means ± SE (n=8). *p< 0.05, Student’s t test. (D) WM3734 melanoma cells at a concentration of 5 × 105 cells/ mouse in 0.1mL Matrigel® were subcutaneously injected into NOD/SCID IL2Rγnull mice (n=8). Mice were monitored and the tumor size was measured twice per week.