Skip to main content
. Author manuscript; available in PMC: 2011 Nov 30.
Published in final edited form as: Biochemistry. 2010 Nov 3;49(47):10072–10080. doi: 10.1021/bi100885v

Table 1.

Enzyme activities of the E. coli MC4100 NDH-1 and various NuoC segment mutants

Strain dNADH-K3Fe(CN)6
activitya 		% dNADH oxidase
activityb 		% dNADH-DB
activityb 		%
Wild type 1816 ± 8 100 659 ± 6 100 636 ± 41 100
KO-C 662 ± 9 36 6 ± 1 1 16 ± 2 3
KO-C-rev 1746± 64 96 831 ± 22 126 674 ± 58 106
graphic file with name nihms250234t1.jpg E138A 890 ± 49 49 11 ± 1 2 18 ± 4 3
E138Q 798 ± 14 44 134 ± 8 20 185 ± 7 29
E138D 925 ± 41 51 29 ± 1 4 41 ± 2 6
E140A 763 ± 26 42 32 ± 3 5 43 ± 1 7
E140Q 604 ± 6 33 45 ± 10 7 68 ± 12 11
E140D 1718 ± 63 94 562 ± 1 85 664 ± 32 104
D143A 903 ± 28 50 10 ± 5 2 22 ± 3 3
D143N 1252 ± 40 69 36 ± 1 5 58 ± 3 9
D143E 1863 ± 12 103 868 ± 11 132 816 ± 14 128
S104A 1870 ± 17 103 699 ± 1 106 727 ± 15 114
A134S 1489 ± 92 82 n.d. n.d.
R139A 1579 ± 12 87 671 ± 9 102 752 ± 17 118
G146A 1856 ± 3 102 771 ± 3 117 724 ± 17 114
F149A 1979 ± 68 109 n.d. n.d.
R156A 2250 ± 1 124 864 ± 5 131 781 ± 29 123
G166A 2061 ± 24 113 868 ± 7 132 741 ± 19 117
H167A 1683 ± 16 93 485 ± 6 74 614 ±13 97
P168A 1652 ± 68 91 494 ± 33 75 534 ± 53 84
K171A 1385 ± 14 76 466 ± 12 71 508 ± 3 80
K171R 1455 ± 17 80 515 ± 3 78 557 ±9 88
P182A 1819 ± 16 100 470 ± 10 71 560 ± 68 88
a

Activity in nmol of K3Fe(CN)6 reduced/mg of protein/min

b

Activity in nmol of dNADH oxidized/mg of protein/min

n.d., not determined.