GFP-fusion constructs containing the FH1-FH2 domains of FRL2 (A, FH1-FH2 construct), mDia2 (B, FH1-FH2-C construct) or FRL1 (C, FH1-FH2-C construct) were expressed in Jurkat cells for six hours, followed by formaldehyde fixation and staining with rhodamine-phalloidin and DAPI. Panels A-C represent cells that were allowed to adhere to poly-L-lysine coated coverslips prior to fixation. Panel D represents cells that were transfected with FRL2 FH1-FH2-C construct, then fixed in suspension prior to adhesion to poly-L-lysine coated coverslips. Scale bar represents 5 μm.