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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov 2;67(12):755–772. doi: 10.1002/cm.20485

Figure 2. FRL2 and mDia2 FH1-FH2 domain-containing constructs induce filopodia in 300.19 cells.

Figure 2

GFP-fusion constructs containing the FH1-FH2 domains of FRL2 (A, FH1-FH2), mDia2 (B, FH1-FH2-C) or FRL1 (C, FH1-FH2-C) were expressed in 300.19 cells for six hours, followed by formaldehyde fixation and staining with rhodamine-phalloidin and DAPI. Panels A-C represent cells that were allowed to adhere to poly-L-lysine coated coverslips prior to fixation. Panel D represents cells that were transfected with FRL2 FH1-FH2-C then fixed in suspension prior to adhesion to poly-L-lysine coated coverslips. Scale bars represent 5 μm.