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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov 2;67(12):755–772. doi: 10.1002/cm.20485

Figure 3. Dynamics of FRL2 FH1-FH2 induced filopodial protrusion in HeLa cells.

Figure 3

HeLa cells were transfected with GFP-FRL2 FH1-FH2 (green) and mRFP-utropin-CH (red, a gift from Bill Bement) to label actin filaments. Micrographs were acquired at 10 sec intervals as Z-series and maximum intensity projections generated. The montage shows selected frames from Movie 3. Two filopodia actively protrude in this sequence. The white arrows mark the initial positions of the GFP-enriched filopodial tips, and remain in the same positions in subsequent frames to show progressive elongation. Times are given in sec.