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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Cytoskeleton (Hoboken). 2010 Nov 2;67(12):755–772. doi: 10.1002/cm.20485

Figure 7. Effects of additional domains on FRL2-induced filopodia.

Figure 7

Jurkat cells were electroporated with GFP-fusion constructs of varying lengths of FRL2, then fixed and stained with rhodamine-phalloidin. A) Bar diagram of FRL2, showing the lengths of each construct. The FH1-FH2 construct is 504-945, FH1-FH2-C is 504-1027, DeltaN45 is 46-1027, Full is 1-1027, and ΔDAD.is 1-987. Domain boundaries are: GBD - 45-236; DID - 79-395; DD (dimerization domain) 398-452; FH1 - 505-548; FH2 - 561-945; DAD - 991-1000. The basis for the boundary predictions is given in Materials and Methods. B) - D) are micrographs of individual cells transfected with each construct. See Table 1 for quantification of phenotype. E) Filopodial lengths for the three constructs tested. Values for minimum and maximumlengths (in μm) and number of filopodia measured are as follows. FH1-FH2: 0.3 μm, 7.5 μm, and n= 109. FH1-FH2-C: 0.6, 9.4, and 70. DeltaN45: 0.6, 11.6, and 115. Scale bars represent 5 μm.