Table 1.

Ability of transfected constructs to induce filopodia

Cell Line	Formin	Construct	% Induction	N	Figure
GFP Fusion Consructs
Jurkat	None	--	0	75	S1
Jurkat	FRL2	FH1-FH2	84	67	1, 6, 7
Jurkat	FRL1	FH1-FH2-C	0	46	1
Jurkat	mDia2	FH1-FH2-C	86	22	1
Jurkat	mDia1	FH1-FH2-C	0	27	S1
Jurkat	FHOD	FH1-FH2	0	23	S1
Jurkat	FRL2	FH1-FH2 I649A	0	33	7
Jurkat	FRL2	FH1-FH2-C	80	20	7
Jurkat	FRL2	DeltaN45*	73	15	7
300.19	None	--	0	50	S2
300.19	FRL2	FH1-FH2	97	34	2
300.19	FRL1	FH1-FH2-C	0	34	2
300.19	mDia2	FH1-FH2-C	63	35	2
300.19	mDia1	FH1-FH2-C	0	41	S2
300.19	FHOD	FH1-FH2	0	49	S2
3T3	None	--	0	12	S3
3T3	FRL2	FH1-FH2	93	14	S3
3T3	FRL1	FH1-FH2-C	0	16	S3
3T3	mDia2	FH1-FH2-C	89	9	S3
3T3	mDia1	FH1-FH2-C	0	16	S3
3T3	FHOD	FH1-FH2	0	17	S3
Chimeric GFP Fusion Constructs
Jurkat	FRL1	FH2	0	48	---
Jurkat	FRL2	FH2	0	39	---
Jurkat	FRL1/FRL1	FH1/FH2	0	54	4
Jurkat	FRL1/FRL2	FH1/FH2	75	48	4
Jurkat	FRL2/FRL1	FH1/FH2	0	51	4
Jurkat	FRL2/FRL2	FH1/FH2	83	47	4
Untagged Constructs
Jurkat	FRL2	Full-length	38	32	8
Jurkat	FRL2	Delta-DAD	82	33	8

% induction refers to % of cells displaying at least five prominent filopodia, with GFP enrichment at tips. For 3T3 cells, % induction refers to % transfected cells showing enriched GFP staining at filopodial tips.

^*DeltaN45 contains the GBD/DID/dimerization domain/FH1/FH2/and C-terminus.

N refers to number of cells counted.

Results are representative of at least three experiments.

For Jurkats and 300.19 cells, cells were adhered to coverslips before fixation. However, where tested, similar results were obtained when cells were fixed prior to coverslip mounting.