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. Author manuscript; available in PMC: 2011 Nov 23.
Published in final edited form as: Vaccine. 2010 Oct 8;28(50):8015–8025. doi: 10.1016/j.vaccine.2010.08.106

Fig. 3. NS-LAIVs Induce IFN and ISGs in Human Cells.

Fig. 3

(A) Confluent A549 cells were inoculated with the recombinant viruses at an MOI of 4 TCID50/cell. Supernatants were collected at 8, 16 and 24 hpi, UV-inactivated, transferred to naive A549 cells, and incubated for 24h. The treated A549 cells were subsequently inoculated with VSV-GFP (MOI=2) and GFP expression in cells was visualized by fluorescence microscopy at 4 hpi. (B) RT-PCR analysis of IFN-β and GAPDH mRNAs in virus infected cells at 24hpi. (C-F) Confluent A549 cells were inoculated with the recombinant viruses at MOI of 4. Total cellular RNA was extracted from the inoculated cells at 4, 8, 12, 16 hpi and the levels of IFN-β (C), IL-29 (IFN-λ (D)), IP-10 (E) and MxA (F) mRNA were determined by quantitative RT-PCR. Error bars indicate the SEM of triplicate assays.