Male C57BL/6 mice were treated daily with vehicle (open circles), 50 mg lopinavir/12.5 mg ritonavir/kg (50 mg/kg L/R, closed triangles), or 100mg lopinavir/25 mg ritonavir/kg (100 mg/kg L/R, closed squares) and were evaluated regularly for (A) body weight; (B) total body fat mass expressed as percent fat mass measured at day 0; and (C) food intake. All data are mean and S.E.M. of 9–18 mice per group. Data were analyzed by 2-way ANOVA, and *, **, and *** indicate significant (p<0.05, p<0.01, and p<0.001, respectively) differences noted in lopinavir/ritonavir-treated mice as compared to vehicle-treated mice at the same timepoint.