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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Biochem Pharmacol. 2010 Sep 15;81(1):24–31. doi: 10.1016/j.bcp.2010.09.001

Figure 4.

Figure 4

Analysis of CE expression in human lung and kidney microsomes.

A - Graph indicating the levels of CE activity (black bars) and SN-38 production (white bars) from individual kidney microsomal samples.

B – Graph indicating the levels of CE activity (black bars) and SN-38 production (white bars) by both pooled and individual lung microsomal samples. NS – non smokers; S – smokers.

C – Western analysis of hiCE and hCE1 expression in individual human kidney microsomal extracts. Purified hiCE and hCE1 protein (50ng) were included as positive controls.

D – Western analysis of hiCE and hCE1 expression in pooled human lung microsomal extracts isolated from 4 individuals. Purified hiCE and hCE1 protein (50ng) were included as positive controls. As before, NS – non smokers; S – smokers.

E – Coomassie blue stained gel demonstrating equal loading of the samples analyzed in panel C.

F – Prolonged exposure of a western analysis using larger amounts (200μg) of lung microsomal extracts following immunoblotting with hiCE antibody. L - human liver microsomes.

G – Coomassie blue stained gel demonstrating equal loading of the samples analyzed in the western analyses. As above, NS – non smokers; S – smokers.