Table 3.
Effect of various extracts of M. elengi bark on antioxidant enzymes in renal tissue
| Treatment | Catalase activity (A/mg protein) | Superoxide dismutase activity (B/mg protein) | GSH (nmoles/mg protein) | MDA (nmoles/mg protein) |
|---|---|---|---|---|
| Normal | 3.13 ± 0.05 | 9.03 ± 0.15 | 3.64 ± 0.14 | 3.82 ± 0.26 |
| Ethylene glycol | 0.94 ± 0.03a | 3.90 ± 0.15a | 0.48 ± 0.15a | 9.96 ± 0.54a |
| Cystone (750 mg/kg b.w.) | 2.83 ± 0.18*** | 5.47 ± 0.17*** | 2.53 ± 0.18*** | 4.12 ± 0.32*** |
| Petroleum ether extract (200 mg/kg b.w.) | 1.12 ± 0.04ns | 4.27 ± 0.16ns | 0.94 ± 0.24ns | 8.62 ± 0.36 |
| Chloroform extract (200 mg/kg b.w.) | 1.25 ± 0.11ns | 3.81 ± 0.16ns | 0.89 ± 0.18ns | 8.47 ± 0.53ns |
| Alcohol extract (200 mg/kg b.w.) | 2.46 ± 0.08*** | 6.08 ± 0.19*** | 1.87 ± 0.14*** | 5.10 ± 0.38*** |
Data were analysed by ANOVA followed by Dunnett’s test. Each value is the mean ± SEM; n = 6;
a
P < 0.001 when compared to normal group;
***
P < 0.001 when compared to ethylene glycol treated group; A, Micro mole of H2O2 consumed per minute; B, one unit of activity was taken as the enzyme reaction, which gave 50% inhibition of NBT reduction in 1 min; MDA, malondialdehyde; GSH, glutathione.