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. 2010 Nov 10;10:133. doi: 10.1186/1471-230X-10-133

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Copyright ©2010 Eckhardt et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SAA levels in plasma and stool of knockout and wildtype mice. Samples of acute phase plasma, obtained 24 h after intravenous injection of 1 microgram LPS into wildtype mice or DKO mice (A), or extracts of stool samples from wildtype mice (B) were analyzed by immunoblotting with anti SAA1/2 antiserum following SDS-PAGE. The right lane in "B", labeled "SAA", is a positive control consisting of acute-phase plasma obtained as described for panel A. The reduced mobility of SAA in stool samples is likely caused by self-aggregation of SAA.