Table 2.
Classification of GFP-expressing Embryos from Transient expression Experiments.
| Reporter construct | Floor plate | Notochord | Expressing embryos | Injected embryos | Freq. | ||
|---|---|---|---|---|---|---|---|
| <15 cells | >15 cells | <15 cells | >15 cells | N° | N° | % | |
| 0.8shh:gfp | 0 | 0 | 0 | 0 | 22 | 102 | 22 |
| 2.4shh:gfp | A: 0 P: 164 |
A: 171 P: 7 |
0 | 0 | 171 | 234 | 73 |
| lar-D:0.8shh:gfp | A: 0 P: 46 |
A: 182 P: 136 |
0 | 0 | 182 | 268 | 68 |
| 0.8:shh:gfp:lar-A | 0 | 0 | 0 | 123 | 123 | 167 | 74 |
| 0.8shh:gfp:lar-B | 3 | 0 | 0 | 0 | 31 | 143 | 22 |
N° GFP-positive cells per embryo of each construct in the expression domains of interest. The abundance of GFP-expressing embryos below and above the threshold is shown (15 GFP-positive cells per embryo). Abbreviations: A: anterior floor plate; P: posterior floor plate.