Skip to main content
. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Wiley Interdiscip Rev Syst Biol Med. 2010 Aug 20;3(2):167–182. doi: 10.1002/wsbm.108

Figure 8. Measuring DNA binding of a transcription factor.

Figure 8

The levels of NF-κB binding to a target promoter could be measured by tagging NF-κB with GFP (green) and observing its binding to a tandem gene array. Such an array already exists for NF-κB and promoters containing κB consensus motifs or HIV LTR, and similar design principles could be used to construct an array cell line for any transcription factor. An array is composed of a series of NF-κB target sites (red rectangle) each followed by a reporter gene (blue). The array is stably integrated at a chromosomal locus. Live cells containing the GFP-tagged NF-κB (green circles) exhibit a bright green spot in the nucleus due to clustering of NF-κB sites at the tandem array. The brightness of this spot is proportional to the amount of NF-κB binding to the target site.