Table 2.
Primer sequences, fluorescent dyes, groups simultaneously used for multiplex PCR, repeat motifs and accession numbers of the 10 microsatellite markers developed for Neoseiulus womersleyi
| Locus | Forward primera | Reverse primer | Groupb | Repeat motifc | Accession no |
|---|---|---|---|---|---|
| NwMS801 | D3-CCTACCGTTAACCTGGCGTA | GAAAGCGTGAGGAGTGGAAC | C | (CT)16 | AB533197 |
| NwMS810 | D2-GGATGAAGAGAGAGCGAGAAAGTAT | ACCTCCATTTTCTTCCTCCTT | A | (AG)11 | AB533198 |
| NwMS814 | D4-CGCGAGCGAGCTTGTTTT | GTCCTCTTCCGATCAACACC | D | (CT)23 | AB533199 |
| NwMS828 | D4-TTCATCTCTCGACCCTCTCC | GGAGGAAACTAGGAGCTGGA | B | (TC)9 | AB533200 |
| NwMS831 | D2-CAGAGAACGAGAAGAGATCAGG | CATCGTCAGACTTTGTTCCTGT | B | (GA)8 | AB533201 |
| NwMS856 | D3-CTGGAGCCCCTCGAAGTTTA | GGGCTCGAAAGGTTCAAAA | C | (CT)12 | AB533202 |
| NwMS861 | D4-TTCGTGAAATTCGTTGATCG | AGTGACGATTTCGCCTCAAA | C | (TTTCTCTC)26 | AB533203 |
| NwMS867 | D2-TTCGTCGTCTGTGGAAGTTG | AGCGCAATCGCTTCAAAGT | D | (CT)10 | AB533204 |
| NwMS872 | D4-ATGGCGATACGACGACAAA | CGCTCGCTGAACTCAAATAG | A | (GA)24 | AB533205 |
| NwMS880 | D2-CAAGTTTCCAGCTCGGTCAT | GCAGAAGGAGCTACTGAAGCA | D | (CT)23 | AB533206 |
aBeckman dyes were at the 5′ end
bLoci with same character are simultaneously amplified in the same PCR reaction
cFrom the sequenced clones