Table 6.
The effect of different treatments with the colchicine solution on polyploid induction in S. flavescens
| Number of treated buds | Duration of immersion (h) | Number of surviving buds | Death rate (%) | Number of tetraploids | Tetraploid rate (% of surviving buds) | Tetraploid rate (% of initial buds) |
|---|---|---|---|---|---|---|
| 50 | 0 | 50 | 0.0 | 0 | 0.0 | 0.0 |
| 50 | 12 | 48 | 4.0 | 2 | 4.2 | 4.0 |
| 50 | 24 | 42 | 16.0 | 4 | 9.5 | 8.0 |
| 50 | 36 | 35 | 30.0 | 7 | 20.0 | 14.0 |
| 50 | 48 | 31 | 38.0 | 14 | 45.2 | 28.0 |
| 50 | 60 | 25 | 50.0 | 10 | 40.0 | 20.0 |
| 50 | 72 | 18 | 64.0 | 8 | 44.4 | 16.0 |
| 50 | 84 | 15 | 70.0 | 5 | 33.3 | 10.0 |
| 50 | 96 | 4 | 92.0 | 1 | 25.0 | 2.0 |
Treated buds were cultured for 20 days on a solid medium supplemented with 1.0 mg/l BAP for bud proliferation