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. 2010 Sep 7;285(49):37953–37963. doi: 10.1074/jbc.M110.180018

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 7. — Dimerization of SIRPα is not required for binding to CD47. Serial dilutions of cell supernatants containing known concentrations of soluble CD47 fused with alkaline phosphatase (CD47AP) were assessed in binding assays to cell surface-expressed SIRPα in tunicamycin-treated (Tunica.) or non-treated control (No Tx) CHO-SIRPα cells grown in 96-well tissue culture plates. Analysis of binding curves for affinity constant (K_d) and maximal binding value (B_max) was performed with PRISM software. Data are representative of a quadruplicate set of determinations from an experiment that is representative of one of three independent experiments.