FIGURE 8.

Hsp90 inhibitor binds to TeHsp90 and cures infection in mice. A, determination of binding affinity of GA toward TeHsp90 by tryptophan fluorescence. The change in fluorescence (ΔF) was plotted against GA concentration for TeHsp90. B, GA-immobilized beads specifically pull down TeHsp90 from T. evansi lysate. The GA pulldown fraction was analyzed by SDS-PAGE (upper panel) and two-dimensional electrophoresis (lower panel). C and D, in vivo efficacy of Hsp90 inhibitors on the survivability of T. evansi-infected mice. C, plot of number of parasites against the number of days postinfection. D, percentage of viability of 17AAG-treated/untreated mice plotted against time. A.U., arbitrary units. Error bars have been represented to indicate standard error from three independent experiments.