FIGURE 7.

HS/Heparin disaccharide analysis of peritoneal lavage fluids and cells from naïve and T. gondii infected mice. The HS/heparin pool of GAGs isolated (Fig. 5) was characterized for disaccharide components by extensive heparin lyase digestion as described under “Experimental Procedures” and analyzed by reversed phase ion pairing chromatography and fluorescent detection. Disaccharides were quantified against standard disaccharides and different species plotted as percent of total disaccharides recovered for the lavage (A) and cell fraction (B) of the low salt lavage. The disaccharide species created by lyase treatment are indicated: ΔHexA-GlcNAc (NAc), ΔHexA-GlcNS (NS), ΔHexA-GlcNAc6S (6S), ΔHexA2S-GlcNAc (2S), ΔHexA-GlcNS6S (NS6S), ΔHexA2S-GlcNS (NS2S), and ΔHexA2S-GlcNS6S (NS6S2S), where ΔHexA indicates a 4,5-unsaturated hexuronic acid created by action of the lyase, and S indicates a sulfate group in the indicated position of the respective sugar. In the insets, the overall number of nonsulfated (NonS), N-sulfated (NS), 6-O-sulfated (6S), and 2-O-sulfated (2S) disaccharides per 100 disaccharides and the overall degree of sulfation (TotalS) are indicated.